Database : HANSEN Search on : POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA [Subject descriptor] References found : 7 [refine] Displaying: 1 .. 7   in format [Detailed]

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Id: 17278 Author: Kim, Se-Kom; Lee, Seong-Beom; Kang, Tae-Jin; Chae, Gue-Tae Title: Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR ..- Source: s.l; s.n; 2003. 6 p. ilus, tab. Abstract: The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU). Descriptors: DNA Bacteriano Resistência Bacteriana a Múltiplas Drogas Hansenostáticos Hanseníase Mycobacterium leprae Reação em Cadeia da Polimerase Polimorfismo (Genética) Polimorfismo Conformacional de Simples Fita Análise de Sequência de DNA Limits: Humano Coréia (geográfico) Mutação Puntual Location: BR191.1; 09178/s

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Id: 13953 Author: Kim, Se -Kon; Lee, Seong- Beom; Kang, Tae -Jin; Chae, Gue -Tae Title: Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR ..- Source: s.l; s.n; 2003. 6 p. ilus, tab. Abstract: The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU). Descriptors: DNA BACTERIANO/quim DNA BACTERIANO/genet Resistência Bacteriana a Múltiplas Drogas/genet COREIA (GEOGRAFICO) HANSENOSTATICOS/farmacol HANSENIASE/quimioter HANSENIASE/microbiol MYCOBACTERIUM LEPRAE/genet MUTACAO PUNTUAL REACAO EM CADEIA DA POLIMERASE/métodos POLIMORFISMO (GENETICA) POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUÊNCIA DE DNA Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 09096/s

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Id: 12298 Author: Dequeker, Jan; Fabry, Guy; Vanopdenbosch, Ludo Title: Hieronymus Bosch (1450-1516): paleopathology of the medieval disabled and its relation to the Bone and Joint Decade 2000-2010 ..- Source: s.l; s.n; Nov. 2001. 8 p. ilus, tab. Descriptors: DAPSONA DIIDROPTEROATO SINTASE RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUENCIA DE DNA Limits: ANIMAL Location: BR191.1; 08668/s

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Id: 12278 Author: Kang, Tae-Jin; Chae, Gue-Tae Title: Detection of toll-like receptor 2 (TLR2) mutation in the lepromatous leprosy patients ..- Source: s.l; s.n; 2001. 6 p. ilus, tab. Descriptors: SEQUENCIA DE AMINOACIDOS HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE HANSENIASE TUBERCULOIDE LEUCOCITOS MONONUCLEARES LEUCOCITOS MONONUCLEARES GLICOPROTEINAS DE MEMBRANAS GLICOPROTEINAS DE MEMBRANAS DADOS DE SEQUENCIA MOLECULAR MUTAÇAO MYCOBACTERIUM LEPRAE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA RECEPTORES DA SUPERFICIE CELULAR RECEPTORES DA SUPERFICIE CELULAR ALINHAMENTO DE SEQUENCIA TRANSDUÇAO DE SINAL Location: BR191.1; 08630/s

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Id: 12256 Author: Lee, S. B; Kim, S. K; Kang, T. J; Chae, G. T; Chun, J. H; Shin, H. K; Kim, J. P; Ko, Y. H; Kim, N. H Title: The prevalence of folP1 mutations associated with clinical resistance to dapsone, in mycobacterium leprae isolates from South Korea ..- Source: s.l; s.n; 2001. 4 p. ilus, tab. Descriptors: DAPSONA DIIDROPTEROATO SINTASE RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUENCIA DE DNA Limits: ANIMAL Location: BR191.1; 08670/s

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Id: 11564 Author: Lee, S. B; Kim, S. K; Kang, T. J; Chee, G. T; Chun, J. H; Shin, H. K; Kim, J.P; Ko, Y. H Title: The prevalence of folP1 mutations associated with clinical resistance to dapsone, in Mycobacterium leprae isolates from South Korea ..- Source: s.l; s.n; 2001. 4 p. ilus, tab. Descriptors: DAPSONA DIIDROPTEROATO SINTASE RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE MUTAÇAO MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA ANALISE DE SEQUENCIA DE DNA Limits: ANIMAL Location: BR191.1; 08404/s

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Id: 10213 Author: Wit, Madeleine Y. L. de; Klatser, Paul R Title: Mycobacterium leprae isolates from different sources have identical sequences of the spacer region between the 16S and 23S ribosomal RNA genes ..- Source: s.l; s.n; 1994. 5 p. ilus. Descriptors: TATUS SEQUENCIA DE BASES CERCOCEBUS ATYS DNA BACTERIANO DNA RIBOSSOMICO GENES ESTRUTURAIS BACTERIANOS HANSENIASE HANSENIASE CAMUNDONGOS DOENÇAS DOS MACACOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO (GENÉTICA) POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA RNA RIBOSSOMICO 16S RNA RIBOSSOMICO 23S MAPEAMENTO POR RESTRIÇAO HOMOLOGIA DE SEQUENCIA DO ACIDO NUCLÉICO Location: BR191.1; 06914/s

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